JetBiofill - ELISA Plates
Specification: 96-well non-removable plate 96-well removable plate (fitted with 8-well strip or 12-well strip) Binding Force: High binding force Moderate binding force
Product Information
As an important tool for ELISA (enzyme linked immunosorbent assay) experiments, ELISA plates are made of PS. Antigens, antibodies and biomolecules are binding on the bottom surface of the plate by means of hydrophobic and ionic bonds. The plates are mainly used in disease and food safety diagnosis.
The ELISA plates from Jet Biofil are made with internationally advanced surface treatment technologies and manufacturing processes for high polymer materials, and show stable protein-binding properties. They can be used as safe, reliable and effective carriers during ELISA experiments, and in conjunction with immune and genetically modified products, as well as for clinical diagnosis.
Features
* Unique surface treatment process for higher protein adsorption properties
* 2 binding forces available: High binding force (300~400ng/cm²), and moderate binding force (200~300ng/cm²)
* 8-well and 12-well strips are provided to match the ELISA plates and for better cost-effectiveness
* Designed with a flat bottom and divided into removable and non-removable structures, to satisfy different experimental applications
* Even well diameter and thickness, ensuring high experimental accuracy and repeatability
* Transparent plate, with a CV value <5%, higher measurement flexibility, widely used in colorimetric determination
* Clearly marked with letters and numbers, to distinguish the samples in different wells
* Dimensions conform to SBS international standards, and are stable for most brands of ELISA equipment
* Sterilized by irradiation, SAL 10-6
* DNase/RNase-free, non-pyrogenic
High binding force ELISA plate
The plate undergoes surface treatment to increase protein binding force, up to 300~400ng/cm² (IgG); molecular weight of binding protein: >10kD. This type of ELISA plate can improve sensitivity and reduce the coing protein concentration and usage. If they were not present, the non-ionic detergent to fail to blocking the binding protein, nonspecific reactions could occur, meaning the protein would need to be used as a blocking agent.
Moderate binding force ELISA plate
The ELISA plate binds with proteins through hydrophobic bonds on the surface, and is suitable for use as a solid phase carrier to macromolecule proteins with a molecular weight >20kD, protein binding capability is 200-300ng /cm²(IgG). As the ELISA plate only displays macromolecular binding, it is also suitable as a solid phase carrier for unpurified antibodies or antigens. Proteins or non-ionic detergents can be used as blocking liquid on these plates.
| Type of ELISA Plate | Transmittance Variation (CV) | Binding Action | Sample Characteristics | Recommended Sealing Agent |
| High binding force plate | <5.00% | Hydrophobic bond | Middle/macromolecular protein with positive charge >10kD | PBS containing 0.3% Tween 20, combination of 0.05% Tween 20 and 1%BSA |
| 300 ~ 400ng/cm² | ||||
| （IgG） | ||||
| Moderate binding force plate | Hydrophobic bond/ionic bond | Macromolecular protein >20KD | Detergent Tween 20 is used in combination with protein, BSA, skim milk and serum |