NZY FFPE gDNA Isolation Kit

NZY FFPE gDNA Isolation Kit is designed for the efficient and rapid small-scale preparation of highly pure genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples.

This method utilizes a silica-based spin column and eliminates the need for flammable and aromatic substances such as xylene or d-limonene, which are typically used for deparaffinization. The procedure using in this kit avoids the challenging step of removing organic solvents from tissue pellets, which are often difficult to visualize.

The kit employs optimized lysis buffers containing Proteinase K and SDS, enabling effective tissue digestion through an efficient two-phase process. In the first step, the paraffin in FFPE tissue sections is dissolved using the Buffer NPD. The tissue is then digested with a Proteinase K to solubilize the fixed material, releasing DNA into the solution. A subsequent heat incubation step with a specially formulated buffer efficiently removes crosslinks from the released DNA. Next, the ethanol is added to the lysate, which is loaded onto the NZYSpin FFPE Column. After the DNA is absorbed into the silica membrane, two washing steps ensure the removal of salts, proteins, metabolites, and other cellular contaminants. Finally, pure genomic DNA is eluted, ready for downstream applications such as sequencing, PCR, multiplex-PCR, genotyping and other enzymatic manipulations.