NZY Mag Blood DNA Isolation Kit

The NZY Mag Blood DNA Isolation Kit utilizes paramagnetic bead-based technology to enable the rapid and efficient purification of high-purity genomic DNA from 200 µL of whole blood samples, whether fresh or frozen, and treated with EDTA or citrate.

The method is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions, ensuring maximum DNA yield and purity while effectively removing PCR inhibitors. This kit uses optimized lysis buffer combined with Proteinase K to efficiently lysis whole blood and release DNA from cells. Following the lysis step, DNA is selectively bound to magnetic beads by adding NZY Mag Binding Buffer NBL2 to improve binding conditions. Subsequently, magnetic separation is performed, and multiple wash steps eliminate impurities such as proteins and salts. No drying step is required, as residual ethanol from previous wash steps is removed by using the NZY Mag Wash Buffer NBL4. Finally, pure DNA is eluted using NZY Mag Elution Buffer, yielding ready-to-use DNA suitable for downstream applications. The eluted genomic DNA has an A260/A280 ratio between 1.7 and 1.9 what makes it ready to use in a wide range of downstream applications, including qPCR, isothermal amplification techniques (such as LAMP, HDA, RPA and NASBA), digital PCR, genotyping and other molecular biology assays.

The NZY Mag Blood DNA Isolation Kit is optimized to isolate up to 2-8 µg of pure genomic DNA from 200 µL of whole blood. The DNA concentration can vary between 10–160 ng/μL, depending on the donor's health condition and the elution volume selected.

For enhanced scalability and flexibility, the kit supports manual protocols and automated workflows on platforms like the KingFisher™ Flex (Thermo Fisher Scientific™) and TANBead Nucleic Acid Extractor Maelstrom Switch 8 (Taiwan Advanced Nanotech Inc.). These systems enable the processing of up to 96 samples simultaneously, minimizing preparation time for reagents, and delivering faster and reproducible results. The optimized automation protocols allow the processing of 96 samples within 45 to 120 minutes. By combining cutting-edge technology, user-friendly protocols, and unparalleled efficiency, the NZY Mag Blood DNA Isolation Kit sets a new standard in genomic DNA extraction. It empowers researchers with speed, simplicity, and reliable performance, accelerating scientific discoveries and innovation.