NZY Mag Plant DNA Isolation Kit

The NZY Mag Plant DNA Isolation Kit utilizes paramagnetic bead-based technology to enable the rapid and efficient purification of high-purity genomic DNA from up to 20-50 mg wet weight of plant tissues. The method is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions, ensuring maximum DNA yield and purity while effectively removing PCR inhibitors.

This kit uses optimized lysis buffer based on the established CTAB method to efficiently lysis samples and release DNA from cells. Following the lysis step, DNA is selectively bound to NZY Mag Binding Beads IV by adding NZY Mag Binding Buffer NP2 to improve binding conditions. Subsequently, magnetic separation is performed, and multiple wash steps with wash buffers NP3, NP4 and 80% ethanol eliminate impurities such as proteins and salts. No drying step is required, as residual ethanol from previous wash steps is removed by using the NZY Mag Wash Buffer NP5. Finally, pure DNA is eluted using low salt NZY Mag Elution Buffer, yielding ready-to-use DNA suitable for downstream applications. The eluted genomic DNA has an A260/A280 ratio between 1.6 and 1.9 which makes it ready to use in a wide range of downstream applications, including qPCR, isothermal amplification, digital PCR, genotyping and other molecular biology assays.

NZY Mag Plant DNA Isolation Kit is designed for the simple and rapid manual and automated small-scale preparation of highly pure DNA using static magnetic stands or automated workflows on platforms like the KingFisher™ Flex (Thermo Fisher Scientific™) and TANBead Nucleic Acid Extractor Maelstrom Switch 8 (Taiwan Advanced Nanotech Inc.). These systems enable the processing of up to 96 samples simultaneously, minimizing preparation time for reagents, and delivering faster and reproducible results. The optimized automation protocols allow the processing of 96 samples within 45 to 120 minutes (excluding lysis step). By combining cutting-edge technology, user-friendly protocols, and unparalleled efficiency, the NZY Mag Plant DNA Isolation Kit sets a new standard in genomic DNA extraction. It empowers researchers with speed, simplicity, and reliable performance, accelerating scientific discoveries and innovation.