Paragon AgriType® Auto Targeted Library Kit, 1-Pool Molecular breeding genotyping by sequencing

Genotyping by Sequencing, also known as GBS, is a revolutionary method used for discovering novel plant and animal single nucleotide polymorphisms (SNPs) and performing genotyping studies. Molecular breeding utilizes DNA markers (SNPs) that are tightly linked to phenotypic traits to assist in selection to deliver improved plant and animal species for agricultural use. As major drivers like population growth, urbanization and economic development feed into an ever-complex agricultural landscape, GBS and agricultural genomics have taken a central role to offer sustainable solutions for the global food challenge.

Among the many genotyping platforms, such as PCR- and array-based methods, Next-Generation Sequencing (NGS) has proven to be an exceptional tool for the discovery, validation, and assessment of genetic markers. NGS offers high multiplex levels, robust call rates, and an economical approach to genotyping large numbers of SNPs and samples. Targeted genotyping by sequencing (tGBS) methods such as amplicon sequencing can focus on thousands of markers to provide a cost-effective, high-throughput genotyping solution that is customizable, flexible, and scalable.

Amplicon sequencing is a quick and easy targeted sequencing method with a simple workflow and fast turnaround time, as compared to hybrid-capture sequencing. It has naturally higher on-target rates than other targeted sequencing methods due to the precision of primer design. 

Some of the key genotyping by sequencing applications include:

• Marker-assisted selection (MAS)
• Marker-assisted backcrossing (MABC)
• Genomic selection (GS)
• Trait mapping and screening
• Quantitative trait locus (QTL) screening
• Genetic linkage analysis
• Germplasm or GMO characterization
• Infectious disease identification
• Parentage testing

AgriType™ Targeted Genotyping by Sequencing Solution

Cost-Effective Solution to Accelerate Molecular Breeding

The AgriType™ Targeted Genotyping by Sequencing Solution is designed and developed to address large-scale genotyping projects in agrigenomics (the application of genomics in the field of agriculture). The AgriType™ solution is powered by an advanced primer design algorithm and an efficient ultra-high multiplex PCR-based target enrichment chemistry. AgriType genotyping by sequencing provides a cost-effective solution to construct NGS libraries to quickly and efficiently screen hundreds to tens of thousands of markers of interest to accelerate genotyping studies in fields such as aquaculture, livestock farming, and seed breeding.

• Scalable High-Throughput Genotyping System. Target thousands of relevant markers in the same assay.
• Fast Turnaround Time. Get custom assays delivered in 4 to 6 weeks. Use AgriType genotyping solution to accelerate your large-scale molecular breeding programs. Our PhD-level expert scientists are here to provide speedy and comprehensive support throughout the design, ordering, and technology adoption process, including excellent after-sales technical support.
• Cost-Effective Solution. Process thousands of samples at a time on Illumina® platform. We support 6144 Illumina combinatorial indexes for high-throughput workflows.
• Minimizes consumable consumption. This single-plate workflow reduces consumable cost and loss of material from material transfer.
• Streamlined, Automation-Friendly Workflow. Generate sequencing-ready libraries in just 3 hours using a simple, three-step protocol that can be easily automated.
• High-Quality, Reproducible Results. Prepare high-quality NGS libraries with high marker call rates and efficient use of sequencing reads.

High-Quality GBS Amplicon Library Powered Proprietary Primer Design and Chemistry

The AgriType genotyping solution is powered by our proprietary multiplex PCR chemistry (amplicon) and highly advanced primer design algorithm. Platform-specific index primers are used to generate AgriType™ target-enriched amplicon libraries. We support up to 6144 Illumina combinatorial indexes for high-throughput workflows. Custom panels can be designed for your crop of interest in as little as 2 weeks and all the materials you need to generate high quality targeted NGS libraries are shipped to your lab in another 2-4 weeks. Our technical support team will support your initial testing for high performance right out of the box.